Journal: Integrative biology : quantitative biosciences from nano to macro

Article Title: Vascular Smooth Muscle Contractility Depends on Cell Shape

doi: 10.1039/c1ib00061f

Figure Lengend Snippet: Microcontact printing used to provide spatial cues for vascular tissue self-organization. (A) Phase contrast images of VSMCs micropatterned into thin tissue lines of varying widths, indicated in each image, separated by 100 μm gaps. (scale bars =200 μm). (B) 3-D confocal image of 100 μm wide patterned VSMC tissue. (Green: f-actin, Blue: nuclei). Scale bar 100 μm. (C) Thickness map of tissue in B, using f-actin staining. (D) Tissue thickness as a function of pattern width and ECM (fibronectin (FN) and laminin (LN)) substrate (mean +/− SD).

Article Snippet: Human umbilical artery vascular smooth muscle cells (VSMCs) were purchased from Lonza (Walkersville, MD) at passage 3 and cultured in growth medium consisting of M199 culture medium (GIBCO, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Invitrogen), 10 mM HEPES (GIBCO, Invitrogen, Carlsbad, CA), 3.5 g/L glucose, 2mg/L vitamin B-12, 50 U/ml penicillin and 50 U/mL streptomycin (GIBCO).

Techniques: Staining

Journal: Bioactive Materials

Article Title: A “built-up” composite film with synergistic functionalities on Mg–2Zn–1Mn bioresorbable stents improves corrosion control effects and biocompatibility

doi: 10.1016/j.bioactmat.2023.02.004

Figure Lengend Snippet: (A) Fluorescent staining of HUVECs cultured for 1 day, 3 days, and 5 days. (B) HUVECs' viability after 1 day, 3 days, and 5 days of incubation is calculated from CCK-8 tests. (C) Fluorescent staining of HUASMCs cultured for 1 day, 3 days, and 5 days. (D) HUASMCs' viability after 1 day, 3 days, and 5 days of incubation is calculated from CCK-8 tests. Data are presented as mean ± SD (n = 4), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: In the cell culture experiment, human umbilical vein endothelial cells (HUVECs) and human umbilical artery smooth muscle cells (HUASMCs) purchased from Guangzhou Geneo Biotech were first subcultured and then seeded in 24-well plates at a density of 1.5 × 10 4 and 2 × 10 4 cells/ml, respectively.

Techniques: Staining, Cell Culture, Incubation, CCK-8 Assay

Journal:

Article Title: Development of Composite Porous Scaffolds Based on Collagen and Biodegradable Poly(ester urethane)urea

doi:

Figure Lengend Snippet: Growth curves for scaffolds made from PEUU (C0/ 1080) and PEUU with 10% collagen (C10/1080) seeded with umbilical artery smooth muscle cells and cultured for up to 28 days. The relative cell number is based upon MTT absorption and reflects mitochondrial activity.

Article Snippet: Smooth Muscle Cell Culture Scaffolds were cut and sterilized by immersion in 70% ethanol for 6 h, followed by rinsing with PBS and exposure to the ultraviolet light source in a laminar flow hood (Class II A/B3 Biological Safety Cabinet) for 2 h. Scaffolds were placed in a centrifuge tube with human umbilical artery smooth muscle cells (UASMCs, Bio Whittaker) at a density of 1 × 10 6 cells/ml in medium (SmGM-2 with 10% fetal bovine serum, BioWhittaker).

Techniques: Cell Culture, Activity Assay

Journal:

Article Title: Development of Composite Porous Scaffolds Based on Collagen and Biodegradable Poly(ester urethane)urea

doi:

Figure Lengend Snippet: Electron micrographs of PEUU and PEUU/collagen (90:10) scaffold surfaces after 28 days of culture with umbilical artery smooth muscle cells.

Article Snippet: Smooth Muscle Cell Culture Scaffolds were cut and sterilized by immersion in 70% ethanol for 6 h, followed by rinsing with PBS and exposure to the ultraviolet light source in a laminar flow hood (Class II A/B3 Biological Safety Cabinet) for 2 h. Scaffolds were placed in a centrifuge tube with human umbilical artery smooth muscle cells (UASMCs, Bio Whittaker) at a density of 1 × 10 6 cells/ml in medium (SmGM-2 with 10% fetal bovine serum, BioWhittaker).

Techniques: